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Progen Biotechnik density gradient centrifugation with lymphoprep
Density Gradient Centrifugation With Lymphoprep, supplied by Progen Biotechnik, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lymphoprep+density+gradient+centrifugation/lymphoprep/pm42166452-48-8-13
Average 86 stars, based on 1 article reviews
density gradient centrifugation with lymphoprep - by Bioz Stars, 2026-09
86/100 stars

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Isolation:

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. .. Co-cultures or only PBMCs were treated with the following reagents: PBMC alone with γ-IR (0–4 Gy) delivered using the Gammacell 1000 Elite radiation machine (Nordion International, and the PBMC-RA-FLS co-cultures with 4-OOH IFA (0–34 μM) obtained from Niomech IIT GmbH, and H2O2 (0–160 μM) purchased from Sigma-Aldrich.

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture.
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. ..

Article Title: HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 ( cis -imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas
Article Snippet: .. Patient-derived and normal donor peripheral blood mononuclear cells (PBMCs) were isolated by LymphoPrep density gradient centrifugation (ProGen Biotechnik GmbH, Germany). ..

Centrifugation:

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. .. Co-cultures or only PBMCs were treated with the following reagents: PBMC alone with γ-IR (0–4 Gy) delivered using the Gammacell 1000 Elite radiation machine (Nordion International, and the PBMC-RA-FLS co-cultures with 4-OOH IFA (0–34 μM) obtained from Niomech IIT GmbH, and H2O2 (0–160 μM) purchased from Sigma-Aldrich.

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture.
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. ..

Red Blood Cell Lysis:

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. .. Co-cultures or only PBMCs were treated with the following reagents: PBMC alone with γ-IR (0–4 Gy) delivered using the Gammacell 1000 Elite radiation machine (Nordion International, and the PBMC-RA-FLS co-cultures with 4-OOH IFA (0–34 μM) obtained from Niomech IIT GmbH, and H2O2 (0–160 μM) purchased from Sigma-Aldrich.

Article Title: Sublethal DNA damage switches off B cell effector programs in an RA-FLS-PBMC co-culture.
Article Snippet: .. PBMCs were isolated by Lymphoprep density-gradient centrifugation (Progen), followed by red blood cell lysis, then washed, counted, and added to RA-FLS at a 1:5 ratio (1 RA-FLS:5 PBMCs) in the same medium. ..

Gradient Centrifugation:

Article Title: Age and CMV-Infection Jointly Affect the EBV-Specific CD8 + T-Cell Repertoire
Article Snippet: .. Peripheral blood mononuclear cells were obtained by Lymphoprep (Progen) density gradient centrifugation from heparinized blood, according to the manufacturer's instructions. ..

Article Title: Latent CMV Infection Is Associated With Lower Influenza Virus-Specific Memory T-Cell Frequencies, but Not With an Impaired T-Cell Response to Acute Influenza Virus Infection
Article Snippet: .. Peripheral blood mononuclear cells were obtained by Lymphoprep (Progen) density gradient centrifugation from heparinized blood, according to the manufacturer’s instructions. ..

Article Title: HDM2 antagonist MI-219 (spiro-oxindole), but not Nutlin-3 ( cis -imidazoline), regulates p53 through enhanced HDM2 autoubiquitination and degradation in human malignant B-cell lymphomas
Article Snippet: .. Patient-derived and normal donor peripheral blood mononuclear cells (PBMCs) were isolated by LymphoPrep density gradient centrifugation (ProGen Biotechnik GmbH, Germany). ..



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